Multiple Detector Approaches to Protein Aggregation by SEC

Protein aggregation, where molecules assemble into dimers, trimers, tetramers, or larger multimers, is a common occurrence that can have devastating consequences. Biotherapeutic proteins, such as monoclonal antibodies, can give rise to severe immunological events as a result of aggregation. Therefore, quantitation and monitoring using size exclusion chromatography (SEC) are paramount during manufacture and lot-release QC. This application note considers some of the factors that can influence the degree of aggregation and the importance of optimizing